APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

TUNEL assay cell type - SK-MEL-2 human melanoma

Experiment
TUNEL assay cell type - SK-MEL-2 human melanoma
Product
APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Cell were pretreated with 50 μM, 75 μM, and 100 μM of honokiol for 12, 24, 48, or 72 hours.
Protocol tips
- Cells were fixed with 1% (w/v) paraformaldehyde in PBS and place on ice for 15 minutes
Downstream tips
- If signal intensity is low increase the incubation time for the DNA-labeling reaction

Publication protocol

The DNA fragmentation in SKMEL-2 and UACC-62 cells was quantified using an Apo-BrdU TUNEL assay kit (Molecular Probes, Eugene, OR, USA) per the manufacturer's protocol as reported earlier [17]. Cells were treated with control media, 50 μM, 75 μM, and 100 μM of honokiol for 12, 24, 48, or 72 hours and harvested and fixed shortly after the completion of treatment. DNA nicks in fixed cells were labeled with BrdU; then samples were incubated with Alexa Fluor 488-labeled anti-BrdU antibody. The cells were analyzed using flow cytometry. Both positive and negative controls were run with each assay. The experiment was repeated four times.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SK-MEL-2 human melanoma using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific.

Paper title
Antineoplastic Effects of Honokiol on Melanoma
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU below.

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